Introduction: There was several study and report of occurrence of microorganisms, in particular bacteria on cash banknotes and coins. One of the main source of pathogenic or food poisoning is because of pathogenic bacteria the people commonly carry these bacteria in their nose, mouth, wounds, and intestine, and on their skin. Modern bank notes are made up of special blend of 75% cotton and 25% of linen with small segment of fiber so paper money is something of a in isomer, this formation is of paper money is the potential source, substrate for the growth of the bacteria. As the changes of the hand for the handling of money from the valet, from the underneath of breezier, from the socks causing the carrier of the microorganisms. While from different locations outlets we buy day to day commodities we transfer these microorganisms from one location to another location and transferring diseases specially to the debilated patients and immune compromised patients who are at high risk and vulnerable to get disease. The average life span of low denomination paper banknotes is about 24 months some claims. Methodology:. In this total study 720 samples were taken from different locations of currency of different denominations i.e. from Bank counter 243 sample were taken from ATM Machine 50 samples were taken from Food seller were 94 samples were taken, from Medical store 35 samples were taken from Milk seller 92 samples were taken. from Grocery shop 63 samples were taken, from, Meat shop 80 samples were taken from Road side mechanic 36 samples were taken, from Bus conductor 4 samples were taken and from Beggars 23 sample were taken while study period was from 4.3. 2010 to 31.1. 2011 all the specimens were processed according to standard methods. All the samples were collected in sterile condition and microorganism were isolated according to standard method of BMSI and Laboratory of Jinnah Post Graduate Medical Centre Karachi. Results: This study observed on 720 samples were taken from different locations i.e. from Bank counter 243 and 68 microorganisms were isolated, from 50, ATM Machine 50 samples were taken and 0% microorganism were found, from 94 food seller 94 samples were taken 74 microorganisms were isolated, from Medical store 35 samples were taken 24 microorganisms were isolated, from, Milk seller 92 samples were taken 74 microorganisms were isolated. from Grocery shop 63 samples were taken 38 microorganisms were isolated, from, Meat shop 80 samples were taken 55 microorganisms were isolated from Road side mechanic 36 samples were taken 24 microorganisms were isolated, from Bus conductor 4 samples all samples were positive for isolates and from Beggars 23 sample were taken 19 microorganisms. were isolated. Conclusion: This study observed that the currency circulating in Pakistan is the strong source of the pathogens even the Mycobacterium Tuberculosis and suggested that the people of Pakistan are at the great risk regarding the health point of view.