Objectives: To observe frequency of klebsiellia pneumonia in septicemia newborn Methodology: Study was conducted in Shaheed Muhterma Benazir Bhutto Medical College Karachi with collaboration of Dept. of pediatric Lyari general Hospital (LGH) a tertiary care hospitals of Karachi. Blood samples for culture were collected from 115 newborn admitted in NICU with suspected septicemia. Specimens were inoculated in brain heart infusion broth in ratio of 1:5. Subcultures were plerformed at 24 hours, & 2 hours and on the 7th day. Isolates were identified by standard methods. Result: A total of 77 organisms isolated out of 115 samples. These includes gram negative (50/70) and gram positive (27/77). Klebsiella were 22, Staphylococcus aureus were 13 Staphylococcus epidermidis were 12, Proteus mirabilis 7, Enterobacter 6, pseudomonas 6, Escherichia coli 5, Acinetobacter 3, Citrobacter 2, and Staphylococcus Saprophyticus 1. Conclusion: In the view of Septecemia in neonates klebsiella pneumonia tend to show a higher frequency.