Backgrounds: Coagulase negative staphylococcus often formed as contaminants in clinical specimens e.g. in Swab from the skin, nose, throat, wounds, burns and bedsores, generally their presence is not clinically significant but they sometimes act as opportunistic pathogen and cause infection in the urinary tract or in debliated or immunodeficient subjects, more serious bacteriaemic infection. All staphylococci do not form enterotoxin. In some circumstances, however, coagulase negative staphylococcus (CoNS) may have a pathogenic role, they may cause peritonitis in patients on peritoneal dialysis, chronic septicemia or endocarditis in patients having heart surgery or fitted with an artificial heart valve, meningeal or bacteriamic infection in patients fitted with a ventriculovenous, cerebrospinal fluid shunt and septicemia in immunosuppressed and immunodefective patients. This study was aimed to provide the first insight into distribution of CoNS from infected wounds, to identify the common strains/species of coagulase negative staphylococcus (CoNS) in infected wound and antimicrobial susceptibility testing of coagulase negative staphylococcus (CoNS) isolated from the surgical wards. Wide variety of antibiotic have been used for the treatment of staphylococcal infections and at the same time each family of drugs were introduced, most strains were sensitive to them. Strains resistant to a variety of drugs, drug resistant strains are commonly present in hospital and may present a difficulty in therapy,Where the empericle treatment is only choice. Isolates of coagulase negative staphylococci from hospital sources show a greater frequency and wider spectrum of resistance to different antibiotics other than outside the hospital. Method: In this total study hundred and fifty specimens were taken from surgical ward from June 2006 to December 2006 (75 from wound and 75 from skin), all the specimens were processed according to standard methods. Results: 58 (39%) gram negative rods were isolated 29 (19%) gram positive cocci were isolated. 13 (9%) coagulase negative staphylococcus (CoNS) were isolated among these; 8 (62%) S.epidermidis were isolated, 3 (showed as under. 23%) S.hicus and 2 (15%) S.auricularis were isolated. Conclusion: It has been found from the study that coagulase negative staphylococci (CoNS) are the most common nosocomial organisms, found in hospital: S.hicus and S.auricularis were also isolated other than the S.epidermidis which is most common commensels of the skin and resistant to many antibiotics while vancomycin and tobramycin found the most effective antibiotic.