Objectives: To determine the association between hepatitis B virus (HBV) and hepatitis C virus (HCV) infection and Non Hodgkin's Lymphoma (NHL). Duration & Design: This 2 year observational and analytical, case control study was started on 1 st January 2009 at C.M.C Larkana, after approval of ethical review committee. Methods & Materials: After informed written consent, blood samples from 361 NHL cases and 1444 age and sex matched controls (2 groups) meeting our selection criteria, were sent for detection of HBsAg and Anti-HCV Ab on 3 rd generation ELISA. Pearson's chi-square test was applied to compare HBsAg and Anti-HCV Ab seropositivity in cases and controls and odd ratios were computed using SPSS version 10. Results: 69 (19.1%) of cases, 55 (7.6%) controls in group 1(1 st Degree Relatives) and 60 (8.3%) controls in group 2 (Non Hematological Malignancy) were HBsAg positive, with odd ratios of 2.86 (95% CI; 1.96 - 4.19) for control group 1 and 2.60 (95% CI; 1.79 - 3.78) for control group 2. Beside, 52 (14.4%) of cases, 53 (7.3%) subjects in control group 1and 58 (8.0%) in control group 2 were Anti-HCV Ab positive, with odd ratios of 2.12 (95% CI; 1.41 - 3.18) for control group 1 and 1.92 (95% CI; 1.29 - 2.86) for control group 2. P value was 0.000 for both. Conclusion: HBsAg and Anti-HCV Ab seropositivity is associated with 2-3 times risk of development of NHL.